Genomic Technologies in Diagnostics

Course Outcome:

Upon completion of the course, the participants should be able to:

• CO1: Acquire a comprehensive understanding of diverse molecular techniques utilized in the field of genomic diagnostics, including their principles, applications, and limitations.
• CO2: Develop proficiency in executing key molecular methods, encompassing the extraction, quantification, and quality analysis of nucleic acids, as well as the polymerase chain reaction (PCR), within the context of molecular diagnostics.Module I: Molecular Techniques for Diagnosis. (15 Hours)Analysis of gene expression: Southern blot, Northern blot and western blot. In situ hybridization: FISH & GISH. DNA microarray.  DNA sequencing- Sanger’s, Next Generation Sequencing. DNA fingerprinting and its applications in forensics.Module II: Theory and practical sessions on Molecular techniques: (15 Hours)Basic introduction to molecular diagnosis, Molecular techniques: Nucleic acid extraction methods, Nucleic acid quantification and purity check. Agarose gel electrophoresis. PCR: Principle, procedure, and applications. Types of PCR: Multiplex, nested PCR, RT-PCR.  MODE OF EVALUATION:
  

  FORMATIVE ASSESSMENT FOR THEORY (20 Marks)		SUMMATIVE ASSESSMENT FOR THEORY (30 Marks)
  Components:	Marks	Components:	Marks
  Assignment	10	End exam	30
  MCQ/Quiz	5
  Class Participation	5

   

  Test – 02 & Assignment – 02 (Each Module)