22411 Basic Concepts of Bioanalytics

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About Course

Durartion: 30 Hours

Objective

The objective of this course is to empower students of biology and chemistry with in-dept understanding of basic concepts that form the foundation of any qualitative or quantitative analytical/bioanalytical study.

 Course Outcomes

Upon completion of this course, students will be able to

  1. Explain the concepts related to pH, concentration, and units
  2. Perform calculations to prepare various solutions used in Bioanalytics
  3. Design and perform quantitative experiments.
  4. Analyse and present experimental data

Syllabus

  1. UNIT I – Basics for Quantitative Bioanalysis
    1. Introduction
    2. Safe Laboratory Practices
    3. Density, specific gravity, and percent composition
    4. Dilution, dilution factors, Units, and Interconversion
    5. Molarity and Normality
    6. Acids and bases and Acid- Base Properties of amino acids, pI
    7. pH, pKa and Henderson–Hasselbalch equation
    8. pH meter and preparation of Biological Buffers

    UNIT II – Quantitation

    1. Biochemical calculations
    2. Solvents and their use in biology
    3. Sample preparation
    4. Preparation and storage of reagents
    5. Titration
    6. Spectrophotometric quantitation. Proteins, DNA and RNA
    7. Using Extinction coefficient in bioassays (NAD/NADH)
    8. DNA- melting curve analysis

    UNIT III – Analysis, Interpretation and Presentation of Data

    1. Preparation of calibration curve and calculation of results
    2. Graphs- linear, logarithmic, and exponential
    3. LD50, ED50 and IC50 calculations
    4. LOD and LOQ
    5. Mean, SD, SEM, CV
    6. Presentation of Experimental Data
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