23017_Concepts of Bioanalytics

By Dr. Lyned Dafny Lasrado Uncategorized
Wishlist Share
Share Course
Page Link
Share On Social Media

About Course

Duration: 30 Hours

Objective

The objective of this course is to empower students of biology and chemistry with in-dept understanding of basic concepts that form the foundation of any qualitative or quantitative analytical/bioanalytical study.

 Course Outcomes

Upon completion of this course, students will be able to

  1. Explain the concepts related to pH, concentration, and units
  2. Perform calculations to prepare various solutions used in Bioanalytics
  3. Design and perform quantitative experiments.
  4. Analyse and present experimental data

Syllabus

  1. UNIT I – Basics for Quantitative Bioanalysis
    1. Introduction
    2. Safe Laboratory Practices
    3. Density, specific gravity, and percent composition
    4.  Units, and Interconversion
    5. Molarity and Normality
    6. Acids and bases and Acid- Base Properties of amino acids, pI
    7. pH, pKa and Henderson–Hasselbalch equation
    8. pH meter and preparation of Biological Buffers

    UNIT II – Quantitation

    1. Concentration terms
    2. Inter conversion of concentration terms
    3. Dilution
    4. Units of Biomolecules
    5. Titration
    6. Spectrophotometric quantitation. Proteins, DNA and RNA
    7. Using Extinction coefficient in bioassays (NAD/NADH)
    8. DNA- melting curve analysis

    UNIT III – Analysis, Interpretation and Presentation of Data

    1. Preparation of calibration curve and calculation of results
    2. Graphs- linear, logarithmic, and exponential
    3. LD50, ED50 and IC50 calculations
    4. LOD and LOQ
    5. Mean, SD, SEM, CV
    6. Presentation of Experimental Data

Show More

Course Content

UNIT I – Basics for Quantitative Bioanalysis

  • Introduction
    00:00
  • Safe Laboratory Practices
    00:00
  • Part I – Density, Specific gravity and Percent Composition
    00:00
  • Part II – Density, Specific gravity and Percent Composition
    00:00
  • Part I- Units and Interconversion of Units
    00:00
  • Part II- Units and Interconversion of Units
    00:00
  • Part III Units and Interconversion of units
    00:00
  • Molarity and Normality
    00:00
  • Part I-Acids and bases and Acid- Base Properties of amino acids
    00:00
  • Part II-Acids and bases and Acid- Base Properties of amino acids
    00:00
  • Part III-Acids and bases and Acid- Base Properties of amino acids
    00:00
  • Part I – pH meter and Preparation of Biological Buffers
    00:00
  • Part II-pH, pKa and Henderson–Hasselbalch equation
    00:00
  • Part III-pH, pKa and Henderson–Hasselbalch equation
    00:00
  • Part I-pH, pKa and Henderson–Hasselbalch equation
    00:00
  • Part II – pH meter and Preparation of Biological Buffers
    00:00
  • Part III – pH meter and Preparation of Biological Buffers
    00:00
  • Time (3hrs)

UNIT II-Quantitation

UNIT III : Analysis, Interpretation and Presentation of Data

Student Ratings & Reviews

No Review Yet
No Review Yet