23017_Concepts of Bioanalytics

Duration: 30 Hours

Objective

The objective of this course is to empower students of biology and chemistry with in-dept understanding of basic concepts that form the foundation of any qualitative or quantitative analytical/bioanalytical study.

 Course Outcomes

Upon completion of this course, students will be able to

1. Explain the concepts related to pH, concentration, and units
2. Perform calculations to prepare various solutions used in Bioanalytics
3. Design and perform quantitative experiments.
4. Analyse and present experimental data

Syllabus

1. UNIT I – Basics for Quantitative Bioanalysis
   1. Introduction
   2. Safe Laboratory Practices
   3. Density, specific gravity, and percent composition
   4.  Units, and Interconversion
   5. Molarity and Normality
   6. Acids and bases and Acid- Base Properties of amino acids, pI
   7. pH, pKa and Henderson–Hasselbalch equation
   8. pH meter and preparation of Biological Buffers

   UNIT II – Quantitation

   1. Concentration terms
   2. Inter conversion of concentration terms
   3. Dilution
   4. Units of Biomolecules
   5. Titration
   6. Spectrophotometric quantitation. Proteins, DNA and RNA
   7. Using Extinction coefficient in bioassays (NAD/NADH)
   8. DNA- melting curve analysis

   UNIT III – Analysis, Interpretation and Presentation of Data

   1. Preparation of calibration curve and calculation of results
   2. Graphs- linear, logarithmic, and exponential
   3. LD₅₀, ED₅₀ and IC50 calculations
   4. LOD and LOQ
   5. Mean, SD, SEM, CV
   6. Presentation of Experimental Data